siRNA Transfection Kit back

 

DeliverX™ Transfection Reagent is based on novel "MPG" delivery technology developed at Centre de Recherches en Biochimie Macromol(CNRS) in Monpellier (France) in the laboratory of Dr. F. Heitz and Dr. G. Divita.

 

MPG technology uses virus-derived amphipathic peptides that directly interact with nucleic acid cargos to form nanoparticles (150-200 nm) capable of diffusing through plasma membranes and releasing their contents inside the cell. The mechanism of entry is receptor-independent, involves MPG/lipid membrane interactions and avoids the endocytic pathway, thereby preventing endosomal or lysosomal degradation of cargos.

 

 

MPG peptides can be designed to accommodate specific molecular cargos including siRNAs, single and double strand oligonucleotides, small peptides, morpholino antisense oligonucleotides and plasmids.

 

 

 

 

  

• Novel peptide-based, non-endosomal delivery mechanism
• Efficient delivery of siRNA into difficult-to-transfect cell types
• High cell viability at optimal delivery conditions

 

 

 

DeliverX siRNA reagents are available in two formats: DeliverX siRNA or DeliverX Plus siRNA Transfection Kits.

Regardless of which solution suits your needs, each of these reagents offers unrivalled efficiency, minimal toxicity and a simple workflow.

 

·        DeliverX siRNA Transfection Kit contain the reagents required to efficiently transfect siRNAs into most cell types with minimal cell damage and good reproducibility when following the optimization guidelines provided in the User Manual.

 

·        DeliverX Plus siRNA Transfection Kits contain reagents particularly well-suited for transfection of classically difficult-to-transfect cell types and comes with a certificate of analysis for each lot and validated protocols.

 

 

 

We currently have validated protocols (≥70% mRNA knockdown of GAPDH with ≥70% relative cell viability) for the following cell types:

 

• 3T3-L1 Differentiated mouse adipocytes

• C2C12 Differentiated mouse myotubes  
• C2C12 Undifferentiated mouse myocytes

• RAW 264.7 Mouse macrophage cells

• U87MG Human brain glioblastoma astrocytoma cells

• Astrocytoma cells, human
• NHEK-AD Primary human adult keratinocytes

• THP-1 Human peripheral blood acute monocytic leukemia cells
• HUVEC Human umbilical vein endothelial cells

• MDA-MB-231 Human breast adenocarcinoma cells
• MCF-7 Human breast carcinoma cells

   

• HT29 Human colon carcinoma cells
• SW620 Human colon carcinoma cells
• HepG2 Human hepatocarcinoma cells
• BSMC Human bronchial smooth muscle cells

• A549 Human lung carcinoma cells
• A2780 Human ovarian cancer cells

• ASPC-1 Human pancreatic carcinoma cells     

 

 How it works:

 

 

 

Deliver X siRNA – Evaluation kit:

 

The DeliverX siRNA Transfection Evaluation Kit includes all of the components to enable a new

user to evaluate our DeliverX siRNA solution for efficiency of siRNA transfection and simple assay

workflow for a cell type of interest.

The evaluation kit includes the DeliverX siRNA Transfection Reagents and two proficiency controls, FAM-labeled siRNA Control and Human GAPDH siRNA Control, that enable the user to optimize transfections under controlled conditions.

 

·       FAM-labeled siRNA Control enables visualization (1–4 hours post transfection) of transfection efficiency. This validated siRNA delivery control is a 21-mer, double-stranded, deoxyoligonucleotide.

 

·       Human GAPDH siRNA Control is a validated potent siRNA for functional evaluation of transfection efficiency in human cell types.

 

DOCUMENTS