TF ELISA Kit

Panomics TF ELISA Kits are ideal for identifying transcription factor binding activity to DNA. The ELISA-based kit combines a fast, user-friendly format with a sensitive and specific assay.

 

TF activation can be detected with as little as 0.5 µg of nuclear extract and the assay completed in less than 3.5 hours. The 96-well plate format, with individual strips of 8 wells, is suitable for manual use or high-throughput screening applications.

The technology has many applications including the study of drug potency, inhibitor or activator proteins, and/or protein structure/function in the signaling pathway.

 

 

·         Quantify activation of AP-1, ER alpha, GATA1, GR, HIF-1 alpha, MEF-2, NFkBp50, NFkBp65, p53, PPAR alpha, PPAR gamma, SP-1, STAT1, SMAD-2, SMAD-4, FKHR, EGR-1, HNF-3beta, and E2F-1

·         Detect activation with as little as 0.5 µg of nuclear extract

·         Complete assay in less than 3.5 hours

 

 

How it works:

 

In this illustration, activated NFκB p50 molecules from nuclear extracts bind to an NFκB consensus binding site (NFκB Probe) on a biotinylated oligonucleotide. These oligonucleotides are then immobilized on a streptavidin coated 96-well plate. The NFκB p50, bound to the oligonucleotide, is detected by an antibody directed against NFκB p50. An additional horseradish peroxidase (HRP)-conjugated secondary antibody provides a sensitive colorimetric readout quantified by spectrophotometry.

Panomics' TF ELISA Kits quantify transcription factor activation for a specified TF. Our ELISA-based kits combine a fast, user-friendly format with a sensitive and specific assay. TF ELISA Kits are available separately or combined with our Nuclear Extraction Kit. TF ELISA Kits contain all the materials and reagents required to quantitate the TF activation of up to 96 human nuclear extracts in a 96-well assay format.