Procarta SH2 Domain Plex Assay

Procarta SH2 Domain Plex Assay

SH2 Domains: A key to understanding phosphotyrosine-dependent signal transduction

SH2 domains are one of the many protein domain families that mediate protein-protein interactions in signal transduction. Like other domains, SH2 domains are defined by a conserved region of amino acid residues. The folding characteristics of this sequence of 100-amino acids allow these domains to specifically recognize and bind to phosphotyrosine-containing ligands. Protein-protein interactions involving phosphotyrosines, like those made possible by SH2 domains, are a primary means of recruiting signaling proteins, and thus play a major role in signal transduction.

SH2 domains can be found in enzymes, adaptor proteins, regulatory subunits of signaling proteins, scaffold proteins, and transcription factors. These proteins are integral to the signaling process because they act as adaptors between receptors and downstream signaling molecules, transmitting signals within cells and regulating the kinase activity of specific proteins.

Protein phosphorylation is a major conduit of information for cellular responses, and defects in SH2 domain-dependent signaling are often directly or indirectly shown to be involved in human diseases.

Overview:

Luminex-based assay for multiplexed SH2 Domain Measurement.
Rapid – Profile binding with up to 30 Domains in one assay.
Convenient – easy-to-use kit format includes reagents.
Safe – fluorescent detection, no radioactivity involved.

How it works:

Profiling SH2 Interaction Domains Using the Procarta SH2 Plex Assay

Panomics Procarta SH2 Domain Plex Assay was used to simultaneously profile the phosphotyrosine binding to 30 SH2 in a single assay well. COS-1 cells were serum starved overnight and subsequently stimulated with EGF for 0, 5 and 30 minutes. The level of phosphoprotein binding to the GRB2, CRK, CRKL and P85B-D1 beads was induced with the EGF treatment

Kit format:

Panomics’ Procarta SH2 Domain Kits are available in ¼- and 1-plate 96-well formats for the 30 SH2 Domains listed below. The kits contain all the reagents and assay controls required to measure the SH2 binding domains in cell lysates for the 1 and ¼ plate option.

By Request (customer specified) Procarta SH2 Domain kits are available in 1-plate formats with the ability to mix and match from the 30 SH2 domains. We provide the ability to create any combination of SH2’s to form from a 3-29 plex. The kits contain all the reagents and assay controls required to measure the SH2 binding domains in cell lysate for a single plate assay.

DOCUMENTS

Brochure Luminex Assay | download

Procarta SH2 User Manual | download

Elenco Prodotti

NAME

CODE


30-plex, 1 plate	PC6000
30-plex, 1/4 plate	PC6001
By Request, 10 plex, 1 plate	PC6107
By Request, 11 plex, 1 plate	PC6108
By Request, 12 plex, 1 plate	PC6109
By Request, 13 plex, 1 plate	PC6110
By Request, 14 plex, 1 plate	PC6111
By Request, 15 plex, 1 plate	PC6112
By Request, 16 plex, 1 plate	PC6113
By Request, 17 plex, 1 plate	PC6114
By Request, 18 plex, 1 plate	PC6115
By Request, 19 plex, 1 plate	PC6116
By Request, 20 plex, 1 plate	PC6117
By Request, 21 plex, 1 plate	PC6118
By Request, 22 plex, 1 plate	PC6119
By Request, 23 plex, 1 plate	PC6120
By Request, 24 plex, 1 plate	PC6121
By Request, 25 plex, 1 plate	PC6122
By Request, 26 plex, 1 plate	PC6123
By Request, 27 plex, 1 plate	PC6124
By Request, 28 plex, 1 plate	PC6125
By Request, 29 plex, 1 plate	PC6126
By Request, 3 plex, 1 plate	PC6100
By Request, 4 plex, 1 plate	PC6101
By Request, 5 plex, 1 plate	PC6102
By Request, 6 plex, 1 plate	PC6103
By Request, 7 plex, 1 plate	PC6104
By Request, 8 plex, 1 plate	PC6105
By Request, 9 plex, 1 plate	PC6106
Procarta Cell Lysis Buffer, 20 ml	PC6002

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