Procarta Cytokine Assay back

 

 

 

Procarta^® Protein Profiling Assays enable simultaneous monitoring of inter- and intracellular protein signaling pathways in a 96-well plate format. Panomics is developing a broad family of assays designed to measure protein expression and monitor protein modification/activation states in diverse matrices.

 

Procarta protein assays use the xMAP® technology (Mutli-Analyte Profiling Beads) to enable the detection and quantitation of multiple protein targets simultaneously.

The xMAP system combines a flow cytometer, fluorescent-dyed microspheres (beads), lasers and digital signal processing to effectively allow multiplexing of up to 100 unique assays within a single sample. The Procarta assay kits are compatible with all Luminex and Luminex-based instruments currently available.

 

 

Features and Benefits:

 

Procarta Cytokine Kits include all of the necessary reagents for processing samples from cell culture supernatants. If serum, plasma, bodily fluids, or cell lysates or used, then the appropriate diluent must be ordered.

Procarta Cytokine Standard Diluent Kits contain a single component, species/matrix-specific Standard Buffer, for preparation of antigen standards and dilution of experimental samples (if required). Using the appropriate Standard Buffer ensures optimal recovery and sensitivity of the cytokines being analyzed in a serum, plasma, bodily fluids, or cell lysate matrix.

 

 

 

 

 

We offer over 100 assays available for human, mouse, rat, porcine and non-human primate samples (Cytokines, Chemokines, Growth Factors, MMPs, and more) and these assays have been optimized for many different sample types.

 

 

ü       Optimized for sample type: supernatant, serum, plasma, tissues, bodily fluids.

ü       Assay Buffer is matrixed matched for human, mouse, rat, non-human primates and porcine.

ü       Excellent Standard/Spike recovery for all matrices.

ü       Easy to use: no sample dilutions needed, all 1X dilutions.

ü       Low background with better assay range.

ü       Create your own panel from current list of cytokines.

ü       Complete assay in 3 hours with a limit of detection of 1 pg/ml/cytokine or less.

ü       Certificate of Analysis available for every order.

ü       Highly Competitive Price.

 

 

  

How it works:

 

 

Human Cytokine, Chemokine and Growth Factors:

 

Current List of Off the Shelf Kits and available Plexable Human Cytokines.

You can create your own plex set by selecting the analytes within this panel using our By Request Services.

EGF	IL-1 alpha	IL-12 p(70)	MCSF	sCD40 ligand
ENA-78	IL-1 beta	IL-13	MIG	TGF beta*
Eotaxin	IL-1 ra	IL-15	MIP-1 alpha	TGF alpha
FGF basic	IL-2	IL-17A	MIP-1 beta	TNF alpha
GCSF	IL-4	IL-17F	MIP-3 alpha	TNF beta
GM-CSF	IL-5	IL-20	NGF	VEGF
GRO alpha	IL-6	IP10	PAI-1
HGF	IL-7	ITAC	PDGFBB
IFN alpha	IL-8	Leptin	RANTES
IFN beta	IL-10	MCP-1	Resistin
IFN gamma	IL-12 (p40)	MCP-3	SAA

 *TGF-beta should be run as a single plex assay because the sample must be acid treated for proper detection.

Additional Plexable Analytes:

It is possible to combine any of the above targets with the targets below (includes MMP panel as well).  Please note that it may take additional time to prepare your custom panel.

  

Adiponectin	IL-2 ra	MIF	sFas Ligand
BLC	IL-3	MPO	sRANKL
CTACK	IL-9	PIGF	Survivin/BIRC5
Eotaxin 2	IL-16	SAP	TPO
Eotaxin 3	IL-21	SCF	TRAIL
Fractalkine	IL-22	SCGF-b	VCAM
Ganzyme B	IL-23	SDF-1
ICAM	IL-31	sE-selectin
IFN omega	LIF	sFas

Unique Panels and other analytes:

Human MMP-8 Plex

MMP-1	MMP-8
MMP-2	MMP-9
MMP-3	MMP-12
MMP-7	MMP-13

 

Single Plex Assay Kit

CRP

TGF beta

 

Her-2 and Beta Actin 2 - Plex PC0025

HER-2

Beta-Actin

 

 

Mouse Cytokine, Chemokine and Growth Factors:

 

Current List of available Mouse Cytokines. You can create your own plex set by selecting the analytes within a panel.

 

Adiponectin	IL-4	Leptin	RANTES
BTC	IL-5	LIF	sRANKL
Eotaxin	IL-6	IP-10	TGF beta
G-CSF	IL-9	KC	TNF alpha
GM-CSF	IL-10	LIX	VEGF
IFN gamma	IL-12 p40	MCP-1
IL-1 alpha	IL-12 p70	MCP-3
IL-1 beta	IL-13	MCSF
IL-2	IL-17	MIP-1 alpha
IL-3	IL-23	MIP-2

 *TGF-beta should be run as a single plex assay because the sample must be acid treated for proper detection.

Unique Panels and Plex Sets

 

 

 IFN alpha/beta 2 Plex Kit - PC0024

IFN alpha

IFN beta

 

 Single Plex TGF beta

TGF beta

 

Please note that the IFN alpha and beta 2 plex can only be run as two plex and has modified instructions found on the product insert. 

Please note that TGF beta we recommend ordering TGF beta as a single plex because the sample must be treated to measure the active form. 

 

 Rat Panel:

 

Current List of available Rat Cytokines. You can create your own plex set by selecting the analytes within a panel.

 

Eotaxin	IL-4	IP10	RANTES
G-CSF	IL-5	KC	sRANKL
GM-CSF	IL-6	Leptin	TGF beta*
ICAM	IL-10	MCP-1	TNF alpha
IFN gamma	IL-12 p40	MCP-3	VCAM
IL-1 alpha	IL-12 p70	MIP-1 alpha	VEGF
IL-1 beta	IL-13	MIP-2
IL-2	IL-17	NGF

 

*TGF-beta should be run as a single plex assay because the sample must be acid treated for proper detection.

 

Other Analytes

 

 

 Single Plex Kt for CRP Assay PC0022

CRP

 

 TGF beta Single Plex Kit

TGF beta

Non-Human Primate Cytokine, Chemokine and Growth Factors:

Current List of available Non-human Primates Cytokines. You can create your own plex set by selecting the analytes within a panel.

 

Adiponectin	IFN gamma	IL-12 p(70)	MCP-1	Resistin
PAI-1	IL-1 beta	IL-13	MCP-3	sCD40 Ligand
BLC	IL-2	IL-15	MIF	TGF beta1
Eotaxin	IL-4	IL-17A	MIG	TGF alpha
FGF-Basic	IL-5	IL-17F	MIP-1 alpha	TNF alpha
G-CSF	IL-6	IL-1RA	MIP-1 beta	TNF beta
GM-CSF	IL-8	IL-23	NGF	TRAIL
Granzyme B	IL-10	IP10	PDGF-BB	VCAM
ICAM	IL-12 p40	ITAC	RANTES	VEGF

 Porcine Panel  

IL-1 beta

IL-10

IL-4

IL-6

IL-8

TNF alpha

 

DOCUMENTS