Procarta Cytokine Assay back

 

 

 

Procarta^® Protein Profiling Assays enable simultaneous monitoring of inter- and intracellular protein signaling pathways in a 96-well plate format. Panomics is developing a broad family of assays designed to measure protein expression and monitor protein modification/activation states in diverse matrices.

 

Procarta protein assays use the xMAP® technology (Mutli-Analyte Profiling Beads) to enable the detection and quantitation of multiple protein targets simultaneously.

The xMAP system combines a flow cytometer, fluorescent-dyed microspheres (beads), lasers and digital signal processing to effectively allow multiplexing of up to 100 unique assays within a single sample. The Procarta assay kits are compatible with all Luminex and Luminex-based instruments currently available.

 

 

Features and Benefits:

 

Procarta Cytokine Kits include all of the necessary reagents for processing samples from cell culture supernatants. If serum, plasma, bodily fluids, or cell lysates or used, then the appropriate diluent must be ordered.

Procarta Cytokine Standard Diluent Kits contain a single component, species/matrix-specific Standard Buffer, for preparation of antigen standards and dilution of experimental samples (if required). Using the appropriate Standard Buffer ensures optimal recovery and sensitivity of the cytokines being analyzed in a serum, plasma, bodily fluids, or cell lysate matrix.

 

 

 

 

 

We offer over 100 assays available for human, mouse, rat, porcine and non-human primate samples (Cytokines, Chemokines, Growth Factors, MMPs, and more) and these assays have been optimized for many different sample types.

 

 

ü       Optimized for sample type: supernatant, serum, plasma, tissues, bodily fluids.

ü       Assay Buffer is matrixed matched for human, mouse, rat, non-human primates and porcine.

ü       Excellent Standard/Spike recovery for all matrices.

ü       Easy to use: no sample dilutions needed, all 1X dilutions.

ü       Low background with better assay range.

ü       Create your own panel from current list of cytokines.

ü       Complete assay in 3 hours with a limit of detection of 1 pg/ml/cytokine or less.

ü       Certificate of Analysis available for every order.

ü       Highly Competitive Price.

 

 

  

How it works:

 

 
 

Specifications or our assays

The assays used by our Assay Services Team go through the same rigorous optimization as our by request or custom assays that we ship to our customers worldwide. All of the assays are optimized for sample type such as serum, plasma or tissue lysates. Each individual assay must meet our rigorous metrics. To learn more about our assay qualifications, how our data is analyzed please visit this link.

Limit of Detection

Limit of Quantitation

Accuracy/Recovery

Precision Click here for Example Precision Data(pdf)

Specificity

 

Species Specific Order Forms

• Human Cytokine Ordering Form (doc)**NEW ANALYTES AVAILABLE**
• Mouse Cytokine Ordering Form (doc)
• Rat Cytokine Ordering Form (doc)
• Non-Human Primate Cytokine Ordering Form (doc)
• Canine Cytokine Ordering Form (doc) **NEW** 
• Porcine Cytokine Ordering Form (doc)**NEW**

Plexable Human Cytokine, Chemokine and Growth Factors (54 Analytes)

 

EGF	IL-1 ra/IL1R1	IL-17A/CTLA8	PAI-1/Serpin E1
ENA-78/CXCL5	IL-2	IL-17F/ML-1	PDGF BB
Eotaxin/CCL11	IL-4	IL-20	Rantes/CCL5
FGF basic	IL-5	IP10/CXCL10	Resistin/ADSF
Fractalkine	IL-6	ITAC/CXCL11	SAA
GCSF/CSF-3	IL-7	Leptin/LEP	sCD40 ligand
GM-CSF/CSF-2	IL-8/CXCL8	MCP-1/CCL2	TGF beta1*
GRO alpha/CXCL1	IL-9	MCP-3/CCL7	TGF alpha
HGF	IL-10/CSIF	MCSF/CSF-1	TNF alpha
IFN alpha 2	IL-12 (p40)	MIG/CXCL9	TNF beta
IFN beta	IL-12 (p70)	MIP-1 alpha/CCL3	TRAIL/TNFSF10
IFN gamma	IL-13	MIP-1 beta/CCL4	VEGF-A
IL-1 alpha	IL-15	MIP-3 alpha/CCL20
IL-1 beta	IL-16/LCF	Beta NGF/NGFB

Additional Human Analytes

These 36 analytes can be plexed with the above 54 targets, but it will take additional time to prepare your custom panel. Please call or speak to your local sales manager for price and delivery times.

 

Adiponectin	IFN omega	MIF	sFas/TNFRSF6
BDNF	IL-2RA/CD25	MPO	sFas Ligand
BLC/CXCL13	IL-3	OPG	sRANKL/TNFSF11
C-Peptide	IL-21	PIGF-1	Survivin/BIRC5
CTACK/CCL27	IL-22/IL-TIF	SAP/Pentraxin 2	TNFRI
Eotaxin 2/CCL24	IL-23	SCF/MGF	TNFRII
Eotaxin 3/CCL26	IL-27	SCGF-beta	TPO
Granzyme B	IL-31	SDF-1/CXCL12	VCAM-1
ICAM-1	LIF	sE-selectin	TAT

Current List of Plexable Human Cytokines. You can create your own plex set by selecting the analytes within this panel using our By Request Services. We recommend running CRP, TGF-beta1*, MMP-2, Factor V, and Fibrinogen as a Single Plex because these analytes have different sample preparation protocols or dilution folds.

  Human MMP Panel  

 

MMP-1	MMP-3	MMP-7	MMP-8
MMP-9	MMP-12	MMP-13

Human MMP assays can be ordered as a 1-7 plex. MMPs can be plexed with our Human Cytokine/Chemokine assays but actual performance of the final plex assay may vary from published specifications.

  Human Apo Protein Panel  

 

ApoA1	ApoA2	ApoB
ApoC2	ApoE

  Human Cytochrome Panel  

 

Cyp1A2

CYP2B6

CYP3A2

GAPDH

B2M

  Human Coagulation Panel 7-plex  

 

PAI-1	Protein C	Factor VII	Factor VIII
Factor X	TPA	D-Dimer

  Human Coagulation Panel Single Plex  

 

PAI-1

Protein C

Factor VII

  Additional Human Single Plex Assays

 

CRP	TGF beta1	MMP-2
HER-2	GAPDH	B2m

  Human HER-2 and Beta Actin 2 Plex Assay

 

HER-2

Beta-Actin

Measurement of HER-2 protein in lysate samples. This is a two plex kit including detection of Her-2 and beta-actin.

  Available Mouse Cytokine, Chemokine and Growth Factors

 

Adiponectin	IL-3	IL-17 A	MCP-3
BTC	IL-4	IL-21	MCSF/CSF1
Eotaxin	IL-5	IL-23	MIP-1-alpha
G-CSF	IL-6	IP-10	MIP-2
GM-CSF	IL-9	KC/Gro-alpha	RANTES
IFN gamma	IL-10	Leptin	sRANKL
IL-1 alpha	IL-12p40)	LIF	TGF beta1*
IL-1 beta	IL-12p70	LIX/GCP2	TNF alpha
IL-2	IL-13	MCP-1	VEGF A

Current List of plexable Mouse Cytokines. You can create your own plex set by selecting the analytes within this panel. We recommend running TGF-beta* as a single plex assay because the sample must be acid treated in order to measure the active form. Also, CRP is typically present in high concentrations in serum and plasma and should be properly diluted.

  Mouse IFN beta and IFN alpha 2 Plex Assay  

 

IFN alpha

IFN beta

Please note that this two plex assay has a modified protocol for detection. Please refer to the proudct insert for details when running this assay.

  Additional Mouse Single Plex Assays

 

CRP	TGF beta

  Available Rat Cytokine, Chemokine and Growth Factors

 

Eotaxin	IL-4	IP10	RANTES
G-CSF	IL-5	KC/Gro alpha	sRANKL
GM-CSF	IL-6	Leptin	TGF-beta1*
ICAM-1	IL-10	MCP-1	TNF alpha
IFN gamma	IL-12p40	MCP-3	VCAM-1
IL-1 alpha	IL-12p70	MIP-1 alpha	VEGF-A
IL-1 beta	IL-13	MIP-2/Gro beta
IL-2	IL-17A	b-NGF

Current List of available Rat Cytokines. You can create your own plex set by selecting the analytes within a panel. We recommend running TGF-beta* as a single plex because the sample should be acid treated for proper detection. Also, CRP is typically present in high concentrations in serum and plasma and should be properly diluted.

  Additional Rat Single Plex Assays

 

CRP

TGF beta

  Available Non-Human Primate Cytokine, Chemokine and Growth Factors

 

Adiponectin	IL-4	IL-23	Resistin
PAI-1	IL-5	IP10	sCD40 Ligand
BLC	IL-6	ITAC	TGF-beta1
Eotaxin	IL-8	MCP-1	TGF alpha
FGF-Basic	IL-10	MCP-3	TNF alpha
G-CSF	IL-12p40	MIF	TNF beta
GM-CSF	IL-12p70	MIG	TRAIL
Granzyme B	IL-13	MIP-1 alpha	VCAM-1
ICAM-1	IL-15	MIP-1 beta	VEGF-A
IFN gamma	IL-17A	b-NGF
IL-1 beta	IL-17F	PDGF-BB
IL-2	IL-1RA	RANTES

  Canine Panel  

 

MCP-1	IFN gamma	IL-6	IL-2
IL-10	Il-12p40	IL-8	TGF beta
NGF	TNF alpha	SCF	VEGF

  Porcine Panel  

 

IL-1-beta	IL-10	IL-4	IL-6
IL-8	TNF alpha	IFN gamma	TGF beta

  Specifications

Most of Pamonics' Procarta Human, Mouse and Rat Cytokine Assay Kits have a limit of detection of 1 pg/mL or less, a limit of quantitation of 10pg/ml or less and an assay range over 3 logs. Spike recovery from a low and high dose (200 and 1,000pg/ml) in serum and plasma is between 70-130%.

DOCUMENTS