Pneumococcal Immunity Panel

Upon administration of a pneumococcal vaccine, antibody response can achieve measurable concentrations in 2 to 5 weeks. The efficacy of the vaccination is generally assessed by measuring pre- and post-vaccination antibody titers. A 2-fold increase, or greater, is expected in most adults¹. Published literature considers that immunoglobulin G (IgG) response varies by serotype and can be impacted by age and health status¹ ². There is currently no documented agreement on specific post-vaccination antibody concentrations needed to establish protection from pneumococcal infection.

Assay Description

The Luminex Pneumococcal Immunity Panel utilizes Luminex xMAP® multiplexing technology to provide a quantitative assessment of IgG following administration of a pneumococcal vaccine.

xMAP® Pneumo14 contains the following pneumococcal serotypes:

American Designation	1	3	4	8	9	12	14	19	23	26	51	56	57	68
Danish Designation	1	3	4	8	9N	12F	14	19F	23F	6B	7F	18C	19A	9V

Laboratory developed enzyme-linked immunosorbent assays (ELISA) are used by major reference laboratories and hospital laboratories to measure antibody response to vaccination. Each ELISA measures a single serotype on one plate requiring a total of 14 plates to test 14 serotypes. By contrast the xMAP® Pneumo14 assay allows for the simultaneous measurement of all 14 serotypes from a single patient sample in a single well. This significantly reduces the amount of labor and time to results versus traditional methods.

Comparison of xMAP® Pneumo14 to ELISA.

Inter-Laboratory Reproducibility

Since the assay is usually executed with pre- and post-vaccination samples, reproducibility is of utmost importance. This assay demonstrates superior reproducibility in the most stringent inter-laboratory scenarios. In this example, 80 blinded samples run at two different laboratories showed an average correlation coefficients of 0.96.

Median Correlation Coefficient r² = 0.97

Kit Contents
Microspheres coated with 14 pneumococcal serotype specific antigens
3 Controls: High (CH), Medium (CM), and Low (CL)
6 Standards and 1 Background
Conjugate
Wash Solution
Diluent

Order Information

Product Description	Product Number
xMAP® Pneumo14 (Ten Plate Kit)	BG-PNEU00014-10
xMAP® Pneumo14 (One Plate Kit)	BG-PNEU00014-01

DOCUMENTS

Elenco Prodotti

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	Diax srl via Toscana 2 20060 Vignate (MI) P.iva IT12107160157	Protein DNA Electrophoresis Gene Expression Cytokines Fluorochromes Precast Gel	T +39.02.95.360.619 F +39.02.95.360.992 M info@diax.it Tutti i diritti riservati
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