Peptide Transfection Kit back

 

 

Delivery of peptides into cells has traditionally been difficult to accomplish, both in terms of efficiency and retaining normal cellular function post transfection. With the introduction of the DeliverX Peptide Transfection Kit, it is now possible to reproduce and easily deliver a wide variety of peptides into many cell types. Peptide transfection is being pursued for various applications including modulation of cell signaling pathways for basic research, as potential therapeutic agents and for the study of the consequences of pseudo up-regulation of specific cellular peptides.

 

MPG Technology

 

 

DeliverX Transfection Reagents are based on novel ”MPG“ delivery technology developed at Centre de Recherches en Biochimie Macromoléculaire (CNRS) in Montpellier (France) in the laboratory of Dr. F. Heitz and Dr. G. Divita.

 

 

 

 

MPG technology uses virus-derived amphipathic peptides that directly interact with nucleic acid cargos to form nanoparticles (150-200 nm) capable of diffusing through plasma membranes and releasing their contents inside the cell.

The mechanism of entry is receptor-independent, involves MPG/lipid membrane interactions and avoids the endocytic pathway, thereby preventing endosomal or lysosomal degradation of cargos.

MPG peptides can be designed to accommodate specific molecular cargos including siRNAs, single and double strand oligonucleotides, small peptides, morpholino antisense oligonucleotides and plasmids.

  

• Novel peptide-based, non-endosomal delivery mechanism
• Efficient delivery of a wide variety of peptides into most cell types
• Versatile, form a single transfection complex and dilute to evaluate multiple peptide concentrations
• High cell viability at optimal delivery conditions

  

 

How it works:

 

 

 

 

Inhibition of Cell Proliferation by Cdk2 Peptide Inhibitor

 

 

Cyclin dependent kinase (Cdk) 2 is required at multiple stages for progression through the mammalian cell cycle.

In association with cyclin E, Cdk2 promotes the transition from G1 to S phase, and Cdk2/cyclinA complexes drive cells through S phase. The activities of Cdks are highly regulated by association with cyclins, whose temporal expression throughout the cell cycle is tightly controlled, by phosphorylation or dephosphorylation by kinases and phosphatases, and through the actions of inhibitory proteins such as Ink4 and Cip/Kip.

 

 

 

 

 

These key regulators of mammalian cell proliferation are excellent targets for anticancer agents.

Here we demonstrate that cellular proliferation can be inhibited by transfection of a Cdk2 inhibitory peptide into HeLa cells using the DeliverX Peptide Transfection Reagent.

 

 

 

 

 

DeliverX Peptide Transfection Kits

 

Our DeliverX Peptide Transfection Kit is suitable for transfection of most cell types. DeliverX Peptide Transfection Kits contain the reagents required to efficiently transfect peptides into most cell types with minimal cell damage and good reproducibility when following the optimization guidelines provided in the User Manual.

 

The DeliverX Peptide Transfection Evaluation Kit includes all of the components to enable a new user to evaluate our DeliverX Peptide solution for efficiency of peptide transfection and simple assay workflow for a cell type of interest. The evaluation kit includes the DeliverX Peptide Transfection Kit and two proficiency controls, TAMRA-labeled Peptide Control and a Pre-Formed Peptide Transfection Complex Control, that enable the user to optimize transfections under

controlled conditions.

 

Panomics’ TAMRA-labeled Peptide Control enables visualization (1–4 hours post transfection) of transfection efficiency. This validated peptide delivery control is a 15 amino acid peptide with the TAMRA fluorophore at the carboxyl terminus.

 

Panomics’ Pre-Formed Peptide Transfection Complex Control enables you to test whether the DeliverX Peptide Transfection Reagent is compatible with your cell line or cell type. The Peptide Transfection Complex Control consists of the pre-formed complex of DeliverX Peptide Transfection Reagent and the TAMRA-labeled Peptide Control.

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