Nuclear Extraction Kit

Nuclear Extraction kit

 

 

Prepare nuclear extracts for a variety of applications: Double The Reaction—20 rxns from cells and 10 rxns from tissue.

 

The Panomics Nuclear Extraction Kit is ideal for preparing nuclear extracts for use with Protein/DNA Arrays, TF-TF Interaction Arrays, and electrophoretic mobility shift assays (EMSA).

This kit facilitates the extraction of functional crude nuclear proteins from various cell types or whole tissue.

 

The protocol is optimized for about 10^7 cells (near confluent 100 mm plate or 0.5 g of tissue). First, cells are allowed to swell with hypotonic buffer. Then, the cells are disrupted, the cytoplasmic fraction is removed, and the nuclear proteins are released from the nuclei with a high-salt buffer.

 

Easy-to-use Kit Format

The Nuclear Extraction kit can be used for 20 reactions extracting nuclear extract from tissue culture cells grown on 10cm plates or 10 reactions using tissue. The kit comes complete with the following reagents listed below

·         1X Buffer A

·         1X Buffer B

·         100 mM DTT

·         Protease Inhibitor

·         Phosphatase Inhibitor I

·         Phosphatase Inhibitor II