ArmoredRNA - Quantitative and Qualitative Controls

Armored Controls

Armored RNA^® are Excellent Controls for Molecular Diagnostics

As molecular diagnostics has become part of routine clinical laboratory testing for the diagnosis and monitoring treatment of patients infected with RNA viruses such as HIV, HCV, and enterovirus, etc., there has developed a need for stable controls for clinical viral testing.

In developing RNA-based tests for these viruses, laboratories have incorporated positive controls and/or standards from one of three sources: In vitro transcribed RNA, positive patient specimens or commercially available viral preparations (attenuated/inactivated viruses).

However, none of these formats is ideal because of the following concerns:

Ribonucleases are ubiquitous and can cause RNA degradation
Incompatibility with plasma or serum matrix
Viral preparations can have genetic heterogeneity and lot-to-lot inconsistency
Unstable at ambient temperature
Risks associated with infectious agents
Some viruses are difficult to culture (e.g. HCV)
More difficult to use patient specimens in the U.S. after introduction of new “HIPAA” regulations for protection of patient privacy

To control for potential failures of sample preparation and assay performance in viral RNA testing, novel Armored RNA^® controls were developed. The coat protein of Escherichia coli bacteriophage MS2 was used to package RNA fragments encoding target-specific viral sequences such as HIV and HCV.

The main advantage of Armored RNA^® controls is that the packaged RNA is stable and ribonuclease-resistant in plasma and other matrices. The RNA is compatible with any RNA-based clinical assay after sample extraction.

Armored RNA^® controls are ideal as sample extraction controls because they can be added directly to patient samples without risk of being degraded by nucleases in the sample. Thus, if the target RNA is degraded, or if enzymatic inhibitors are co-purified with the target RNA, then a lower than expected result will be obtained for the control RNA, alerting the laboratorian to a potential problem.

The important benefits offered by Armored RNA^® controls have been recognized by clinical laboratories using the controls within routine clinical testing as well as by major diagnostics companies, which have incorporated Armored RNA^® into in vitro diagnostic products.

Armored Technology: Catalog Products

Quantitative Armored RNA*
Armored RNA Quant - Oncology Targets		Unit Amount
42045	Armored RNA Quant BCR/ABL b3/a2 (5 x 10⁵ copies/mL) Spec sheet	5 x 100 µl
42047	Armored RNA Quant BCR/ABL b2/a2 (5 x 10⁵ copies/mL) Spec sheet	5 x 100 µl
42046	Armored RNA Quant BCR/ABL e1/a2 (5 x 10⁵ copies/mL) Spec sheet	5 x 100 µl
Armored RNA Quant - Enterovirus		Unit Amount
42102	Armored RNA Quant Enterovirus (5 x 10⁵ copies/mL) Spec sheet	5 x 100 µL
Armored RNA Quant - Hepatitis C Virus		Unit Amount
42101	Armored RNA Quant HCV (Genotype 2b) (5 x 10⁵ copies/mL) Spec sheet	5 x 100 µL
Armored RNA Quant Human Immunodeficiency Virus		Unit Amount
42103	Armored RNA Quant HIV (Subtype B) (5 x 10⁵ copies/mL) Spec sheet	5 x 100 µL

Qualitative Armored RNA*
Armored RNA - Human Immunodeficiency Virus		Unit Amount
42000	Armored RNA HIV (Subtype B) Spec sheet	250 µL
42001	Armored RNA HIV (Subtype B) Amplicor® HIV-1 Monitor® Qualified Low Positive Control Spec sheet	Custom Orders Only
42002	Armored RNA HIV (Subtype B) Amplicor® HIV-1 Monitor® Qualified High Positive Control Spec sheet	250 µL
Armored RNA - Hepatitis C Virus		Unit Amount
42004	Armored RNA Hepatitis C Virus (Genotype 1a) Spec sheet	250 µL
42006	Armored RNA Hepatitis C Virus (Genotype 1b) Spec sheet	250 µL
42008	Armored RNA Hepatitis C Virus (Genotype 2a/c) Spec sheet	250 µL
42010	Armored RNA Hepatitis C Virus (Genotype 2b) Spec sheet	250 µL
42011	Armored RNA Hepatitis C Virus (Genotype 2b) Amplicor® HCV Monitor® Qualified Positive Control Spec sheet	Custom Orders Only
42012	Armored RNA Hepatitis C Virus (Genotype 3a) Spec sheet	250 µL
42200	Armored RNA Hepatitis C Virus Panel I (Genotypes 1a, 1b, 2a/c, 2b, 3a) Spec sheet	5 X 250 µl
Armored RNA - Hepatitis G Virus		Unit Amount
42024	Armored RNA Hepatitis G Virus Spec sheet	250 µL
Armored RNA - Norwalk Virus		Unit Amount
42030	Armored RNA Norwalk Virus (Genogroup I) Spec sheet	250 µL
42032	Armored RNA Norwalk Virus (Genogroup II) Spec sheet	250 µL
Armored RNA - West Nile Virus		Unit Amount
42040	Armored RNA West Nile Virus (HNY1999) Spec sheet	250 µL
42041	Armored RNA West Nile Virus (NEN) Spec sheet	Custom Orders Only
42042	Armored RNA West Nile Virus (Cap) Spec sheet	250 µL
Armored RNA - Dengue Virus		Unit Amount
42044	Armored RNA Dengue Virus (Type 1) Spec sheet	250 µL
Armored RNA - Enterovirus		Unit Amount
42050	Armored RNA Enterovirus Spe sheet	250 µL
Armored RNA - SARS		Unit Amount
42090	Armored RNA SARS (BNI-1) Spec sheet	Custom Orders Only
42091	Armored RNA SARS (CoV-NC) Spec sheet	Custom Orders Only

*For research use only. Not for use in Diagnostic Procedures

DOCUMENTS

Elenco Prodotti

NAME

CODE

	Diax srl via Toscana 2 20060 Vignate (MI) P.iva IT12107160157	Protein DNA Electrophoresis Gene Expression Cytokines Fluorochromes Precast Gel	T +39.02.95.360.619 F +39.02.95.360.992 M info@diax.it Tutti i diritti riservati
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