Antibody Arrays back

 

 

 

 

Cytokine Antibody Arrays are an ideal tool for profiling cytokine expression at the protein level in comparison to gene expression assays that measure mRNA levels. Arrays detect only secreted cytokines, which represent the levels of active protein.

 

·        Fast—profile multiple cytokines in one hybridization experiment

·        Easy—all in one system, no additional equipment needed

·        Safe—no radioactivity required

·        Sensitive—detect proteins in the range of pg/ml

 

Panomics offers a variety of arrays for profiling cytokine expression at the protein level—Cytokine Antibody Arrays. These arrays facilitate the simultaneous detection of multiple human or mouse cytokines from a variety of sources, including cell lysates, conditioned media, patient sera, plasma, and urine.

 

We currently offer four of these arrays:

 

Human Versions	Version	Serum / Conditioned Media / Plasma	Cell Tissue/Lysate	Cytokines detected
Human Cytokine Antibody Array	1.0	YES	YES	18
Human Cytokine Antibody Array Expanded	3.0	YES	YES	36
Mouse Version	Version	Serum / Conditioned Media / Plasma	Cell Tissue/Lysate	Cytokines detected
Mouse Cytokine Antibody Array	1.0	YES	YES	18

 

 

 

Principle of the Cytokine Antibody Array Assay

 

The Cytokine Antibody Arrays are based on the sandwich ELISA method for detecting protein. First, a capture antibody specific to a particular cytokine protein is immobilized on the array membrane. Second, sample is incubated with the capture antibody, proteins are given time to bind, and any unbound protein is washed away. Then, a second antibody, known as the detection antibody, is introduced. This biotin-conjugated antibody binds to a second epitope on the protein, creating an antibody “sandwich” around the cytokine.

Through a biotin-streptavidin interaction, an enzyme is used to visualize the bound cytokine protein. The linkage between capture antibody, target protein, and detection antibody offers a high degree of selectivity and sensitivity.

 

 

Easy-to-use kit format:

 

Cytokine Antibody Arrays include 2 or more array membranes; biotinylated anti-cytokine antibodies, blocking, hybridization, and wash solutions; and HRP detection reagents.

 

How it works:

 

·     Incubate the sample with the array membrane. During this time, cytokine proteins present in the sample will selectively bind to the anti-cytokine antibodies immobilized on the array.

·     Add the biotin-labeled detection antibodies to the array membrane. These

anti-cytokine antibodies will bind to any captured proteins, creating an antibody

“sandwich.”

·     Using strepavidin-HRP, visualize the antibody-protein complexes on the array to

determine which active cytokines are present in the sample. Chemiluminescent

signal from the enzyme can be detected using either x-ray film or an imaging

system.

 

 

 

 

Human Cytokine Array 1.0

 

 

 
Human Cytokine Array 3.0

 

Mouse Cytokine Array 1.0

 

 

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