Angiogenesis Antibody Arrays back

 

Angiogenesis is the biological process responsible for the generation of new blood vessels from existing endothelial cells. During tumor growth, angiogenesis is hijacked by cancel cells in order to bring nutrients and oxygen to tumors. Normally, angiogenesis is kept under tight control by pro-angiogenic "activator" molecules and anti-angiogenic "inhibitor" molecules. In the presence of cancer cells, however, the careful balance of these molecules is upset, resulting in increased angiogenic activity.

 

More than two dozen activators and inhibitors have been identified. Tumor growth is sustained by abnormal levels of these angiogenesis-related proteins; the affected proteins vary from one type of cancer to another. To gain a clear picture of the angiogenesis-stimulating pathways used by cancer cells, it is necessary to investigate the levels of these cytokines in tumors.

 

The Human and Mouse Angiogenesis Antibody Arrays allow you to do just that.

With these arrays, you can detect picogram levels of 19 different angiogenesis-related cytokines from biological sources, including cell extracts, tissue lysates, conditioned media, patient sera, and plasma.

Don’t be stuck analyzing one factor at a time — Use the Antibody Array to get a global view of angiogenesis in one assay!

 

·         Fast—detect multiple angiogenesis activator and inhibitor proteins at once

·         Easy—all in one system, no additional equipment needed

·         Safe—no radioactivity required

·         Sensitive—detect proteins in the range of pg/ml

 

 

How it works:

 

 

·     Incubate the sample with the array membrane. During this time, cytokine proteins present in the sample will selectively bind to the anti-cytokine antibodies immobilized on the array.

·     Add the biotin-labeled detection antibodies to the array membrane. These anti-cytokine antibodies will bind to any captured proteins, creating an antibody “sandwich.”

·     Using strepavidin-HRP, visualize the antibody-protein complexes on the array to

determine which active cytokines are present in the sample. Chemiluminescent signal from the enzyme can be detected using either x-ray film or an imaging system.

 

 

 

 

 

 

 

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