xTAG® Chemistry
Multiplex PCR
A multiplexed PCR reaction is performed to amplify the regions of interest in the target human or infectious agent genes.
Exo/SAP treatment of PCR Product
The PCR reaction is treated to remove excess nucleotides and primers.
Multiplex ASPE/TSPE
The PCR reaction is then subjected to a primer extension step that is specific for the allele or the infectious agent that is being analyzed (Allele Specific Primer Extension (ASPE) or Target Specific Primer Extension (TSPE)). The 5’ end of the ASPE or TSPE primers is attached to an xTAG® universal tag sequence.
Universal Array Sorting
The 5’ universal tag sequence is hybridized to the complementary anti-tag sequence coupled to a particular xMAP bead set.
Detection
The hybridized beads are read by the Luminex System and results are analyzed by the data analysis software.
Luminex System Detection
About xTAG® Products
Luminex xTAG® products provide accurate, flexible, low cost microarrays, combining any set of 100 single DNA tests and multiplexing them simultaneously in a single reaction. xTAG® products are conducted on the Luminex xMAP® system, which uses lasers to read color-coded microspheres that attach to specific nucleic acid sequences. xTAG® was formerly known as TAG-IT from TmBioscience.
